On RNA Sequencing

http://www.broadinstitute.org/news/1511

"There is an interesting post on RNA-seq by Dave Delano on Illumina’s blog.
Always impressed by the performances of the combination of sample preparation chemistry and instrument output:

'…using Rapid Run mode on the HiSeq 2500, one can run as many as 24 samples at 25 million paired-end 75 basepair reads (50 million total reads) per sample – a depth and format at which published studies have reported the detection of novel features such as gene fusions and alternative transcripts – in less than 24 hours and in a single run.'

Always frustrated that people forget to mention the time and effort necessary to analyze the results."

http://pgandyou.wordpress.com/2013/10/02/rna-seq/